Pantothenate transport in Escherichia coli
نویسندگان
چکیده
منابع مشابه
Isolation and characterization of Escherichia coli pantothenate permease (panF) mutants.
Mutants of Escherichia coli K-12 defective in the pantothenate permease (panF) were isolated and characterized. The panF mutation resulted in the complete loss of pantothenate uptake and of the ability to use extracellular vitamin for growth. The growth phenotypes of panF panD, panF panB, and panF panC double mutants showed that the cytoplasmic membrane was impermeable to external pantothenate....
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Following a previous report on physicochemical properties, the enzymological properties of a homogeneously purified preparation of pantothenate synthetase were described. The optimum pH was 10.0 and optimum temperature 30 degrees C. The lyophilized enzyme was very stable on standing at -20 degrees C. K+ or NH4+ and Mg2+ were required as activators; other cations examined were inhibitive to vari...
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The present study is concerned with the measurement of the unidirectional K flux in E, coli. Methods are described by means of which a fairly dense suspension of cells may be maintained in a well defined steady-state with respect to the intracellular K concentration and the pH of the medium. The kinetics of K 4~ exchange under these conditions are consistent with the presence of a single intrac...
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Escherichia coli has a specitic active transport system for magnesium which can be studied with the radioisotope 28Mg. The accumulation of 2*Mg is temperature-dependent and inhibited by cyanide, dinitrophenol, and m-chlorophenyl carbonylcyanide hydrazone. The initial rate of uptake of SMg shows typical saturation kinetics with a & = 18 /LM magnesium and a V,,,,, = 0.56 pmole per min per 1012 ce...
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K influx and net K flux have been measured in suspensions of chloramphenicol-arrested Escherichia coli. The rate of K exchange in the steady state was independent of the K concentration of the medium over a 200-fold range. Under a number of experimental conditions the rate of exchange may be considerably increased or decreased without changing the cellular K content. These results show that und...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1985
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.162.3.1156-1161.1985